Genomic study of the Tarim Basin mummies in western China reveals an indigenous Bronze Age population that was genetically isolated but culturally cosmopolitan
In a new study, an international team of researchers has determined the genetic origins of Asia’s most enigmatic mummies - the Tarim Basin mummies in western China. Once thought to be Indo-European speaking migrants from the West, the Bronze Age Tarim Basin mummies are revealed to be a local indigenous population with deep Asian roots and taste for far-flung cuisine.
Aerial view of the Xiaohe cemetery. Credits: Wenying Li, Xinjiang Institute of Cultural Relics and Archaeology
As part of the Silk Road and located at the geographical intersection of Eastern and Western cultures, the Xinjiang Uyghur Autonomous Region has long served as a major crossroads for trans-Eurasian exchanges of people, cultures, agriculture, and languages. Since the late 1990s, the discovery of hundreds of naturally mummified human remains dating to circa 2,000 BCE to 200 CE in the region’s Tarim Basin has attracted international attention due to their so-called ‘Western’ physical appearance, their felted and woven woolen clothing, and their agropastoral economy that included cattle, sheep and goat, wheat, barley, millet, and even kefir cheese. Buried in boat coffins in an otherwise barren desert, the Tarim Basin mummies have long puzzled scientists and inspired numerous theories as to their enigmatic origins.
Excavation of burial M75 at the Xiaohe cemetery. Credits: Wenying Li, Xinjiang Institute of Cultural Relics and Archaeology
The Tarim Basin mummies’ cattle-focused economy and unusual physical appearance had led some scholars to speculate that they were the descendants of migrating Yamnaya herders, a highly mobile Bronze Age society from the steppes of the Black Sea region of southern Russia. Others have placed their origins among the Central Asian desert oasis cultures of the Bactria-Margiana Archaeological Complex (BMAC), a group with strong genetic ties to early farmers on the Iranian Plateau.
To better understand the origin of the Tarim Basin mummies’ founding population, who first settled the region at sites such as Xiaohe and Gumugou circa 2,000 BCE, a team of international researchers from Jilin University, the Institute of Vertebrate Paleontology and Paleoanthropology, the Max Planck Institute for Evolutionary Anthropology, Seoul National University of Korea, and Harvard University generated and analyzed genome-wide data from thirteen of the earliest known Tarim Basin mummies, dating to circa 2,100 to 1,700 BCE, together with five individuals dating to circa 3,000 to 2,800 BCE in the neighboring Dzungarian Basin. This is the first genomic-scale study of prehistoric populations in the Xinjiang Uyghur Autonomous Region, and it includes the earliest yet discovered human remains from the region.
The Tarim Basin mummies were not newcomers to the region
Typical Xiaohe boat coffin with oar. The coffin is covered with a cattle hide. Credits: Wenying Li, Xinjiang Institute of Cultural Relics and Archaeology
To their great surprise, the researchers found that the Tarim Basin mummies were not newcomers to the region at all, but rather appear to be direct descendants of a once widespread Pleistocene population that had largely disappeared by the end of the last Ice Age. This population, known as the Ancient North Eurasians (ANE), survives only fractionally in the genomes of present-day populations, with Indigenous populations in Siberia and the Americas having the highest known proportions, at about 40 percent. In contrast to populations today, the Tarim Basin mummies show no evidence of admixture with any other Holocene groups, forming instead a previously unknown genetic isolate that likely underwent an extreme and prolonged genetic bottleneck prior to settling the Tarim Basin.
“Archaeogeneticists have long searched for Holocene ANE populations in order to better understand the genetic history of Inner Eurasia. We have found one in the most unexpected place,” says Choongwon Jeong, a senior author of the study and a professor of Biological Sciences at Seoul National University.
In contrast to the Tarim Basin, the earliest inhabitants of the neighboring Dzungarian Basin descended not only from local populations but also from Western steppe herders, namely the Afanasievo, a pastoralist group with strong genetic links to the Early Bronze Age Yamanya. The genetic characterization of the Early Bronze Age Dzungarians also helped to clarify the ancestry of other pastoralist groups known as the Chemurchek, who later spread northwards to the Altai mountains and into Mongolia. Chemurchek groups appear to be the descendants of Early Bronze Age Dzungarians and Central Asian groups the from Inner Asian Mountain Corridor (IAMC), who derive their ancestry from both local populations and BMAC agropastoralists.
“These findings add to our understanding of the eastward dispersal of Yamnaya ancestry and the scenarios under which admixture occurred when they first met the populations of Inner Asia,” says Chao Ning, co-lead author the study and a professor of School of Archaeology and Museology at Peking University.
The Tarim Basin groups were genetically but not culturally isolated
A naturally mummified woman from burial M11 of the Xiaohe cemetery. Credits: Wenying Li, Xinjiang Institute of Cultural Relics and Archaeology
These findings of extensive genetic mixing all around the Tarim Basin throughout the Bronze Age make it all the more remarkable that the Tarim Basin mummies exhibited no evidence of genetic admixture at all. Nevertheless, while the Tarim Basin groups were genetically isolated, they were not culturally isolated. Proteomic analysis of their dental calculus confirmed that cattle, sheep, and goat dairying was already practiced by the founding population, and that they were well aware of the different cultures, cuisines, and technologies all around them.
“Despite being genetically isolated, the Bronze Age peoples of the Tarim Basin were remarkably culturally cosmopolitan – they built their cuisine around wheat and dairy from the West Asia, millet from East Asia, and medicinal plants like Ephedra from Central Asia,” says Christina Warinner, a senior author of the study, a professor of Anthropology at Harvard University, and a research group leader at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany.
“Reconstructing the origins of the Tarim Basin mummies has had a transformative effect on our understanding of the region, and we will continue the study of ancient human genomes in other eras to gain a deeper understanding of the human migration history in the Eurasian steppes,” adds Yinquiu Cui, a senior author of the study and professor in the School of Life Sciences at Jilin University.
A profile view of the burial M13 from the Xiaohe cemetery. Credits: Wenying Li, Xinjiang Institute of Cultural Relics and Archaeology
References:
The genomic origins of the Bronze Age Tarim Basin mummies, Nature, DOI: 10.1038/s41586-021-04052-7
Press release from the Max Planck Institute for Evolutionary Anthropology.
Horses were first domesticated in the Pontic-Caspian steppes, northern Caucasus, before conquering the rest of Eurasia within a few centuries. These are the results of a study led by paleogeneticist Ludovic Orlando, CNRS, who headed an international team including l’Université Toulouse III - Paul Sabatier, the CEA and l’Université d’Évry. Answering a decades-old enigma, the study is published in Nature on 20 October 2021.
By whom and where were modern horses first domesticated? When did they conquer the rest of the world? And how did they supplant the myriad of other types of horses that existed at that time? This long-standing archaeological mystery finally comes to an end thanks to a team of 162 scientists specialising in archaeology, palaeogenetics and linguistics.
A few years ago, Ludovic Orlando's team looked at the site of Botai, Central Asia, which had provided the oldest archaeological evidence of domestic horses. The DNA results, however, were not compliant: these 5500-year-old horses were not the ancestors of modern domestic horses1. Besides the steppes of Central Asia, all other presumed foci of domestication, such as Anatolia, Siberia and the Iberian Peninsula, had turned out to be false.
“We knew that the time period between 4,000 to 6,000 years ago was critical but no smoking guns could ever be found” says CNRS research professor Orlando.
The scientific team, therefore, decided to extend their study to the whole of Eurasia by analysing the genomes of 273 horses that lived between 50,000 and 200 years BC. This information was sequenced at the Centre for Anthropobiology and Genomics of Toulouse (CNRS/Université Toulouse III - Paul Sabatier) and Genoscope2 (CNRS/CEA/Université d’Évry) before being compared with the genomes of modern domestic horses.
This strategy paid off: although Eurasia was once populated by genetically distinct horse populations, a dramatic change had occurred between 2000 and 2200 BC.
“That was a chance: the horses living in Anatolia, Europe, Central Asia and Siberia used to be genetically quite distinct” notes Dr Pablo Librado, first author of the study.
Then, a single genetic profile, previously confined to the Pontic steppes (North Caucasus)3, began to spread beyond its native region, replacing all the wild horse populations from the Atlantic to Mongolia within a few centuries.
“The genetic data also point to an explosive demography at the time, with no equivalent in the last 100,000 years” adds Orlando. “This is when we took control over the reproduction of the animal and produced them in astronomic numbers.”
But how can this rapid population growth be explained? Interestingly, scientists found two striking differences between the genome of this horse and those of the populations it replaced: one is linked to a more docile behaviour and the second indicates a stronger backbone. The researchers suggest that these characteristics ensured the animals’ success at a time when horse travel was becoming “global”.
The study also reveals that the horse spread throughout Asia at the same time as spoke-wheeled chariots and Indo-Iranian languages. However, the migrations of Indo-European populations, from the steppes to Europe during the third millennium BC4 could not have been based on the horse, as its domestication and diffusion came later. This demonstrates the importance of incorporating the history of animals when studying human migrations and encounters between cultures.
This study was directed by the the Centre for Anthropobiology and Genomics of Toulouse (CNRS/ Université Toulouse III – Paul Sabatier) with help from Genoscope (CNRS/CEA/Université d’Évry). The French laboratories Archéologies et sciences de l'Antiquité (CNRS/Université Paris 1 Panthéon Sorbonne/Université Paris Nanterre/Ministère de la Culture), De la Préhistoire à l'actuel: culture, environnement et anthropologie (CNRS/Université de Bordeaux/Ministère de la Culture) and Archéozoologie, archéobotanique : sociétés, pratiques et environnements (CNRS/MNHN) also contributed, as did 114 other research institutions throughout the world. The study was primarily funded by the European Research Council (Pegasus project) and France Genomique (Bucéphale project).
1 Read this press release: Unsaddling old theory on origin of horses, 22 February 2018. 2 Genoscope is a department of CEA-Jacob. 3 The Pontic steppe is the western part of the Eurasian steppe. The home of the modern domestic horse is thought to be in the Don and Volga basins, east of the Dnieper. 4 For example, see this press release: 7,000 years of demographic history in France, 25 May 2020.
Press release from CNRS on the study, published on Nature, concerning the origin of domestic horses.
The new study on the origin of domestic horses, references:
Librado, P., (...), Orlando, Ludovic (2021). "The origins and spread of domestic horses from the Western Eurasian steppes". Nature, 20/10/2021. DOI: 10.1038/s41586-021-04018-9
Horses were domesticated in the Northern Caucasus steppes and then spread across Asia and Europe
A large group of researchers have conducted the largest genetic study carried out to date, which has made it possible to determine that the horses from which all current domestic horses descend were first domesticated in the steppes north of the Caucasus and, from there, spread to other regions of Asia and Europe.
Researchers from the Milá y Fontanals Institution (IMF) and the Institute of Archaeology (IAM) of the Spanish National Research Council (CSIC), together with scientists from the Museum of Human Evolution (MEH), the Faculty of Veterinary Medicine of the University of Extremadura (UEx), the UCM-ISCIII Joint Centre for Human Evolution and Behaviour in Madrid, the Laboratory of Prehistoric Archaeology of the University Jaume I of Castellón (UJI) and the Faculty of Geological Sciences of the Complutense University of Madrid (UCM)have participated in the largest genetic study carried out to date, which has made it possible to determine that the horses from which all current domestic horses descend were first domesticated in the steppes north of the Caucasus and, from there, spread to other regions of Asia and Europe.
This study brings to an end a long-standing debate about the location and chronology of the earliest documented evidence of domestication of the horses that gave rise to today's populations, as well as aswering questions about when this domestication process began to spread to other regions of the world, thus replacing other types of horses that existed at the time. The results have been published in the October issue of prestigious international journal Nature.
This conclusion was reached by a team of 114 institutions and 162 researchers specialising in archaeology, palaeogenetics and linguistics, led by Professor Ludovic Orlando, CNRS researcher and principal investigator of the ERC-PEGASUS project, which, together with France Genomique-Projet Bucéphale, financed the research. The study involved sequencing the genomes of 273 remains of horses that inhabited various regions of Eurasia in a chronological arc extending from 50,000 to 200 BC. All the genetic information was sequenced at the Centre for Anthropobiology and Genomics of Toulouse, CAGT (CNRS/University of Toulouse III-Paul Sabatier) and Genoscope (CNRS/CEA/University of Évry), before being compared to the genomes of modern domestic horses. Thanks to the large battery of statistical analyses carried out, it has been possible to establish that between 2,200 and 2,000 BC, a drastic change took place in which the genetic profile existing in the Pontic steppes began to spread beyond its region of origin, thus replacing in a few centuries all wild horse populations from the Atlantic to Mongolia.
According to L. Orlando, "this replacement in the genetic composition of Eurasian populations is associated with significant genomic differences between this new type of horse and the horses of the populations that disappeared. On the one hand, this new type of horse from the steppes of the northern Caucasus had a more docile behaviour and, on the other hand, a more robust constitution in the vertebral skeleton".
The researchers suggest that these characteristics triggered the successful selection of these animals, at a time when horse travel was becoming widespread in Eurasia.
According to Pablo Librado (CNRS), first author of this research, "this study has also shown that the distribution of this new type of horse in Asia coincides with the appearance of light carts and the spread of Indo-Iranian languages. In contrast, the migration of Indo-European populations from the steppe zone to the heart of Europe during the third millennium BC did not use this new type of horse as a vector for its expansion. This result demonstrates the importance of also incorporating the genetic history of animals when analysing the dimension of human migrations and intercultural contacts".
The individuals analysed include equids from various sites on the Iberian Peninsula, including Casas del Turuñuelo (Guareña, Badajoz) and Cova Fosca (Alt Maestrat, Castelló).
The Cova Fosca was excavated by Francesc Gusi and Carmen Olaria. According to C. Olaria, professor of Prehistory at the UJI and co-author of this study,
"Cova Fosca has a very rich Holocene archaeozoological record. We were able to identify horse remains in ancient Neolithic levels, a very rare taxon in Iberian sites from this period. This uniqueness allowed us to publish years ago, together with Jaime Lira Garrido and Juan Luis Arsuaga, the first mitochondrial sequences of horses from this site".
According to J. L. Arsuaga, scientific director of the Museum of Human Evolution, professor of Palaeontology at the UCM, director of the UCM-ISCIII Joint Centre and co-author of this study,
"in Cova Fosca we found a unique mitochondrial lineage exclusive to Iberia that currently appears in very few horses, all of which are Iberian or of Iberian origin. In this new study we aimed to unveil the genomic secrets of the Cova Fosca".
Building Tartessos and Casas del Turuñuelo
A new study on the origin of domestic horses.View of animal slaughter documented in the courtyard of the building at Casas del Turuñuelo
Casas del Turuñuelo is one of the most impressive discoveries in peninsular archaeology in recent years. Its excavations are being carried out under a project directed by the IAM-CSIC and are being co-directed by Esther Rodríguez González and Sebastián Celestino, also researchers at the IAM-CSIC.
According to Esther Rodríguez González, co-author of this new study, "Turuñuelo is an architectural complex from the middle of the first millennium BC belonging to the Tartessos culture where we have found the largest hecatomb documented to date in a site of Mediterranean protohistory. This mass slaughter is notable for the large number of equids that have been differentiated in the courtyard of this site. For this study we selected Equid 4".According to Sebastián Celestino, also co-author of this research, "a multidisciplinary team of specialists from the humanities and biosciences has been created around Turuñuelo, which is generating a constant exchange of information and ideas, thus offering a great multidisciplinary approach to the study of this site".
Among the lines of research of "Construyendo Tartessos" [Building Tartessos], the genetic study of these slaughtered equids stands out. Jaime Lira Garrido (UEx/Centro Mixto UCM-ISCIII), who is a co-author of this study, explains that
"this latest work led by Professor Orlando has also allowed us to delve deeper into the evolutionary history of Iberian horses. In a previous study, Orlando and his team discovered that a genomic lineage developed on the Iberian Peninsula that is now extinct and very different from the rest of the ancient and modern Eurasian horse lineages described to date. The evolutionary origin of this lineage and the causes that led to its disappearance are still unknown. However, we have been able to identify in the Neolithic sample from the Cova Fosca the oldest evidence of this extinct lineage and that the Turuñuelo Equid 4 was, nevertheless, a descendant of this new type of horse that was so rapidly distributed throughout the known world some 4.000 years ago".
This study has been funded mainly by the European Research Council (PEGASUS project) and France Genomique (Bucéphale project).
Press release from Asociación RUVID on the new study on the origin of domestic horses.
Scientists Prove the Northern Caucasus Focus of Horse Domestication for Eurasia
South Ural State University (SUSU) scientists and their foreign colleagues have continued study devoted to the time and place of horse domestication. A research group has established where exactly the focus of domestication is located in Eurasia. The results of the work have been published in the highly rated journal “Nature” (Top 10%).
The identical signs have been found in the genomes of domesticated horses in Eurasia. Specialists have noticed that remains of these animals have a strong spine. Probably, this is the reason for domesticated horses popularity and global spreading of horse travels.
The research group which includes PhD, SUSU professor Andrey Epimakhov has established the Pontic-Caspian steppes, northern Caucasus, is the centre of horse domestication. The process has occurred between 2000 and 2200 BC (the Bronze Age). Horses are distinct i n different presumed foci of domestication such as Anatolia, Siberia and the Iberian Peninsula, but several ages later the common genome has spreaded from the Atlantic to Mongolia within a few centuries.
“Our research group that is led by paleo-geneticist Ludovic Orlando, National Center for Scientific Research (France), has analysed the genomes of 273 horses that lived between 50,000 and 200 years BC. The study has covered the whole of Eurasia to research presumed territories to be considered as primary foci of domestication. However, all of the territories have turned out to be false. For instance, the site of Botai, Central Asia, has provided the oldest archaeological evidence of domestic horses. Nevertheless, the DNA results are not compliant: these 5500-year-old horses are not the ancestors of modern domestic horses. On the contrary, the Ural 4000 years old samples don’t raise doubts in horse exploitation and chariot teams,” Andrey Epimakhov explained.
Research has also demonstrated that the horse spread throughout Asia at the same time as spoke-wheeled chariots and Indo-Iranian languages.
Remind that dental calculus research has helped scientists to determine the time and place of horse domestication. There are dairy diet markers that have been detected in samples found in the Volga region. Milk has been a part of people's ration since animal domestication.
Press release from the South Ural State University on the new study on the origin of domestic horses.
The University of Valencia participates in a research on the evolution of the last ten thousand years of the hepatitis B virus
A study published in the journal Science traces the evolution of the hepatitis B virus from prehistory to the present, revealing dissemination routes and changes in viral diversity. Domingo Carlos Salazar García, researcher from the Prehistory, Archeology and Ancient History Department of the University of Valencia, has participated in this study led by the Max Planck Institute for the Science of Human History (Germany). The research uncovers the evolution of the hepatitis B virus since the Early Holocene by analyzing the largest dataset of ancient viral genomes produced to date.
Domingo Carlos Salazar García, researcher from the Prehistory, Archeology and Ancient History Department of the University of Valencia
“This research puts upfront a reality many times ignored but obvious, that viruses have been linked to humans since prehistoric times”, highlighted Salazar, graduated in Medicine and in History, researcher of excellence of the Valencian Community at the University of Valencia. “If SARS-COV-2 has been able to put human societies in check worldwide during the twenty-first century, we can only begin to imagine how viral diseases influenced life in prehistoric times”, he explains. “Historians and archaeologists must start considering more the influence of viruses and other agents that until now have been invisible on the archaeological record when reconstructing past lifestyles”, he says.
The hepatitis B virus (HBV) is a major health problem worldwide, causing close to one million deaths each year. Recent ancient DNA studies have shown that HBV has been infecting humans for millennia, but its past diversity and dispersal routes remain largely unknown. A new study conducted by a large team of researchers from all around the world provides major insights into the evolutionary history of HBV by examining the virus’ genomes from 137 ancient Eurasians and Native Americans dated between ~10,500 and ~400 years ago. Their results highlight dissemination routes and shifts in viral diversity that mirror well-known human migrations and demographic events, as well as unexpected patterns and connections to the present.
Present-day HBV strains are classified into nine genotypes, two of which are found predominantly in populations of Native American ancestry. The study provides strong evidence that these strains descend from an HBV lineage that diverged around the end of the Pleistocene and was carried by some of the first inhabitants of the Americas.
“Our data suggest that all known HBV genotypes descend from a strain that was infecting the ancestors of the First Americans and their closest Eurasian relatives around the time these populations diverged”, says Denise Kühnert, leader of the research group.
HBV in prehistoric Europe
The study also shows that the virus was present in large parts of Europe as early as 10,000 years ago, before the spread of agriculture to the continent. “Many human pathogens are thought to have emerged after the introduction of agriculture, but HBV was clearly already affecting prehistoric hunter-gatherer populations”, says Johannes Krause, director of the Department of Archaeogenetics at the Max Planck Institute for Evolutionary Anthropology and co-supervisor of the study.
After the Neolithic transition in Europe, the HBV strains carried by hunter-gatherers were replaced by new strains that were likely spread by the continent’s first farmers, mirroring the large genetic influx associated with the expansion of farming groups across the region. These new viral lineages continued to prevail throughout western Eurasia for around 4,000 years. The dominance of these strains lasted through the expansion of Western Steppe Herders around 5,000 years ago, which dramatically altered the genetic profile of Europeans but remarkably was not associated with the spread of new HBV variants.
The collapse and re-emergence of pre-historic HBV
One of the most surprising findings of the study is a sudden decline of HBV diversity in western Eurasia during the second half of the 2nd millennium BCE, a time of major cultural shifts, including the collapse of large Bronze Age state societies in the eastern Mediterranean region.
“This could point to important changes in epidemiological dynamics over a very large region during this period, but we will need more research to understand what happened”, says Arthur Kocher, lead author and researcher in the group.
All ancient HBV strains recovered in western Eurasia after this period belonged to new viral lineages that still prevail in the region today. However, it appears that one variant related to the previous prehistoric diversity of the region has persisted to the present. This prehistoric variant has evolved into a rare genotype that seems to have emerged recently during the HIV pandemic, for reasons that remain to be understood.
Bronze Age Scandinavia's trading networks for copper settled
Crossing the North Sea before crossing the Alps!
Shafthole axe type Fårdrup. This axe is of Nordic craftsmanship and hides information on the first attempt to establish trading networks with societies across the Alps. A small group of these Nordic crafted axes is made of northern Italian copper, so called AATV-copper (from the Alto Adige, Trentino and Veneto mining region in the Italian Alps) while the majority of these axes is made of British and Welsh or eastern Alpine metal. Photo credits: Heide W. Nørgaard, by permission of the National Museum, Copenhagen
New research presents over 300 new analyses of bronze objects, raising the total number to 550 in 'the archaeological fingerprint project'. This is roughly two thirds of the entire metal inventory of the early Bronze Age in southern Scandinavia. For the first time, it was possible to map the trade networks for metals and to identify changes in the supply routes, coinciding with other socio-economic changes detectable in the rich metal-dependent societies of Bronze Age southern Scandinavia.
The magnificent Bronze Age in southern Scandinavia rose from copper traded from the British Isles and Slovakia 4000 years ago. 500 years later these established trade networks collapsed and fresh copper was then traded from the southern Alps, the so-called Italian Alps. This large-scale study could show that during the first 700 years of the Nordic Bronze Age the metal supplying networks and trade routes changed several times. These 700 years of establishment and change led to a highly specialised metalwork culture boasting beautiful artwork such as the Trundholm Sun wagon and spiral decorated belt plates branding high-ranking women; even depicted on today's Danish banknotes.
The lead isotope plot of the over 65 shafthole axes analyzed in this study dating to the end of the first Bronze Age period 1600 BC. This amount of data exceeds the previous analyses by ten times and for the first time allows to compare both axe types and understand their development
The study by H. Nørgaard, Moesgaard Museum and her colleagues H. Vandkilde from Aarhus University and E. Pernicka from the Curt-Engelhorn Centre in Mannheim built on the so far largest dataset of chemical and isotope data of ancient bronze artefacts. In total 550 objects were used to model the changes that took place: These changes correlate with major shifts in social organisation, settlements, housing, burial rites and long distance mobility.
Shafthole axe of Valsømagle type. Only a few axes of this type are known, and they are only distributed in northern Europe. These axes seem to be contemporary with the Fårdrup type axes as they are made of the same metal and not, if they would be slightly later, of the new Italian metal that is the main metal used in the period from 1500 BC. Photo credits: Heide W. Nørgaard, by permission of the National Museum, Copenhagen
"Now, this multi-disciplinary approach - based jointly on conventional archaeological methods and novel scientific methodologies processing large data quantities - allows us to detect these correlating changes and identify contemporaneity with societal changes recognised by colleague researchers", says Heide Nørgaard the project´s PI.
"It is highly likely that both people and technologies arrived to Scandinavia and that Scandinavians travelled abroad to acquire copper by means of the Nordic amber, highly valued by European trading partners".
Ancient Genomes Trace the Origin and Decline of the Scythians
Generally thought of as fierce horse-warriors, the Scythians were a multitude of Iron Age cultures who ruled the Eurasian steppe, playing a major role in Eurasian history. A new study published in Science Advances analyzes genome-wide data for 111 ancient individuals spanning the Central Asian Steppe from the first millennia BCE and CE. The results reveal new insights into the genetic events associated with the origins, development and decline of the steppe’s legendary Scythians.
Because of their interactions and conflicts with the major contemporaneous civilizations of Eurasia, the Scythians enjoy a legendary status in historiography and popular culture. The Scythians had major influences on the cultures of their powerful neighbors, spreading new technologies such as saddles and other improvements for horse riding. The ancient Greek, Roman, Persian and Chinese empires all left a multitude of sources describing, from their perspectives, the customs and practices of the feared horse warriors that came from the interior lands of Eurasia.
Still, despite evidence from external sources, little is known about Scythian history. Without a written language or direct sources, the language or languages they spoke, where they came from and the extent to which the various cultures spread across such a huge area were in fact related to one another, remain unclear.
The Iron Age transition and the formation of the genetic profile of the Scythians
A new study published in Science Advances by an international team of geneticists, anthropologists and archeologists lead by scientists from the Archaeogenetics Department of the Max Planck Institute for the Science of Human History in Jena, Germany, helps illuminate the history of the Scythians with 111 ancient genomes from key Scythian and non-Scythian archaeological cultures of the Central Asian steppe. The results of this study reveal that substantial genetic turnovers were associated with the decline of the long-lasting Bronze Age sedentary groups and the rise of Scythian nomad cultures in the Iron Age. Their findings show that, following the relatively homogenous ancestry of the late Bronze Age herders, at the turn of the first millennium BCE, influxes from the east, west and south into the steppe formed new admixed gene pools.
The study goes even further, identifying at least two main sources of origin for the nomadic Iron Age groups. An eastern source likely originated from populations in the Altai Mountains that, during the course of the Iron Age, spread west and south, admixing as they moved. These genetic results match with the timing and locations found in the archeological record and suggest an expansion of populations from the Altai area, where the earliest Scythian burials are found, connecting different renowned cultures such as the Saka, the Tasmola and the Pazyryk found in southern, central and eastern Kazakhstan respectively. Surprisingly, the groups located in the western Ural Mountains descend from a second separate, but simultaneous source. Contrary to the eastern case, this western gene pool, characteristic of the early Sauromatian-Sarmatian cultures, remained largely consistent through the westward spread of the Sarmatian cultures from the Urals into the Pontic-Caspian steppe.
The decline of the Scythian cultures associated with new genetic turnovers
The study also covers the transition period after the Iron Age, revealing new genetic turnovers and admixture events. These events intensified at the turn of the first millennium CE, concurrent with the decline and then disappearance of the Scythian cultures in the Central Steppe. In this case, the new far eastern Eurasian influx is plausibly associated with the spread of the nomad empires of the Eastern steppe in the first centuries CE, such as the Xiongnu and Xianbei confederations, as well as minor influxes from Iranian sources likely linked to the expansion of Persian-related civilization from the south.
Although many of the open questions on the history of the Scythians cannot be solved by ancient DNA alone, this study demonstrates how much the populations of Eurasia have changed and intermixed through time. Future studies should continue to explore the dynamics of these trans-Eurasian connections by covering different periods and geographic regions, revealing the history of connections between west, central and east Eurasia in the remote past and their genetic legacy in present day Eurasian populations.
Press release from Max Planck Institute for the Science of Human History in Jena on the new study that helps illuminate the history of the Scythians with 111 ancient genomes from archaeological cultures of the Central Asian steppe.
Genetic adaptations of early Italian ancestors to environmental changes, such as those that occurred soon after the Last Glacial Maximum, may explain some of the genetic differences between northern and southern Italian populations today, according to a study published in BMC Biology. The research suggests that northern and southern Italian populations may have begun to diverge genetically as early as 19,000-12,000 years ago and constitutes the earliest known evidence of genetic divergence in Italy so far.
A team of researchers at the University of Bologna sequenced the genomes of 38 unrelated participants from different regions in Italy, each the third generation of their family native to each region. The genomes were selected as representative of known genetic differences across the Italian population and over 17 million distinct genetic variants were found between individuals. The authors compared these variations with existing genetic data from 35 populations across Europe and the Mediterranean and with variants previously observed in 559 ancient human remains, dating from the Upper Palaeolithic (approx. 40,000 years ago) to the Bronze Age (approx. 4,000 years ago).
Prof. Marco Sazzini, lead author of the study said: “When comparing sequences between modern and ancient genome samples, we found early genetic divergence between the ancestors of northern and southern Italian groups dating back to the Late Glacial, around 19,000-12,000 years ago. Migrations during the Neolithic and Bronze Age periods, thousands of years later, then further differentiated their gene pools. Divergence between these ancestral populations may have occurred as a result of temperature rises and subsequent shrinking of glaciers across Northern Italy during this time, allowing ancestors who survived the glaciation period to move north, separating from groups who remained in the south.”
Further analyses also revealed signatures ascribable to specific biological adaptations in northern and southern Italian genomes suggestive of habitation in differing climates. The genetic history of northern Italians showed changes in the genes responsible for regulating insulin, body-heat production and fat metabolism, whilst southern Italians showed adaptations in genes regulating the production of melanin and responses to pathogens.
Prof. Sazzini said: “Our findings suggest that the ancestors of northern Italians adapted to lower environmental temperatures and the related high-calorie diets by optimising their energy metabolism. This adaptation may play a role in the lower prevalence of Type 2 Diabetes recorded in Northern Italy today. Conversely, southern Italian ancestors adapted to a warmer climate with higher UV levels by increasing melanin production, which may explain the lower incidence rates of skin cancers recorded across Southern regions. The genomes of southern Italians also showed changes in the genes encoding mucins, which play a role in protection against pathogens, and genetic variants linked to a longer lifespan. Further research in this area may help us understand how the observed genetic differences can impact population health or predisposition to a number of diseases.”
The authors caution that although correlations may be drawn between evolutionary adaptations and current disease prevalence among populations, they are unable to prove causation, or rule out the possibility that more recent gene flow from populations exposed to diverse environmental conditions outside of Italy may have also contributed to the different genetic signatures seen between northern and southern Italians today.
Adaptive events evolved by ancestors of N_ITA/S_ITA clusters and their health implications for present-day Italians. The putative selective pressures having plausibly prompted local adaptations are displayed on the left, while biological processes subjected to natural selection are reported on the map along with their impact on present-day disease susceptibility. Distribution of biological adaptations having the potential to modulate the longevity phenotype (e.g., involving the mTOR signaling, arachidonic acid metabolism, and FoxO signaling pathways) in the overall Italian population, but especially in people from Southern Italy, is represented by the arrow on the right. Putative selective pressures, biological processes, and distribution of adaptations potentially modulating longevity are color-coded as follows: N_ITA, blue; S_ITA, red. Picture from the paper, credits Sazzini, M., Abondio, P., Sarno, S. et al., CC BY 4.0
Sazzini, M., Abondio, P., Sarno, S. et al. Genomic history of the Italian population recapitulates key evolutionary dynamics of both Continental and Southern Europeans. BMC Biol18, 51 (2020). https://doi.org/10.1186/s12915-020-00778-4
Stable isotope analyses reveals dramatic diet diversification at the onset of the steppe's earliest empires
The historic economies of Mongolia are among the least understood of any region in the world. The region's persistent, extreme winds whisk away signs of human activity and prevent the buildup of sediment which archaeologists rely on to preserve the past. Today crop cultivation comprises only a small percent of Mongolia's food production, and many scholars have argued that Mongolia presents a unique example of dense human populations and hierarchical political systems forming without intensive farming or stockpiling grains.
The current study, led by Dr. Shevan Wilkin of the Max Planck Institute for the Science of Human History provides, for the first time, a detailed glimpse into the diets and lives of ancient Mongolians, underscoring the importance of millets during the formation of the earliest empires on the steppe.
Isotopic analysis and the imperial importance of millets
Mongolian landscape with pastoral herd of sheep and goats. Credits: Alicia Ventresca Miller
Collaborating with archaeologists from the National University of Mongolia and the Institute of Archaeology in Ulaanbaatar, Dr. Wilkin and her colleagues from the MPI SHH sampled portions of teeth and rib bones from 137 previously excavated individuals. The skeletal fragments were brought back to the ancient isotope lab in Jena, Germany, where researchers extracted bone collagen and dental enamel to examine the ratios of stable nitrogen and carbon isotopes within. With these ratios in hand, scientists were able to reconstruct the diets of people who lived, ate, and died hundreds to thousands of years ago.
Researchers tracked the trends in diet through the millennia, creating a "dietscape" which clearly showed significant differences between the diets of Bronze Age peoples and those who lived during the Xiongnu and Mongol Empires. A typical Bronze Age Mongolian diet was based on milk and meat, and was likely supplemented with small amounts of naturally available plants. Later, during the Xiongnu Empire, human populations displayed a larger range of carbon values, showing that some people remained on the diet common in the Bronze Age, but that many others consumed a high amount of millet-based foods. Interestingly, those living near the imperial heartlands appear to have been consuming more millet-based foods than those further afield, which suggests imperial support for agricultural efforts in the more central political regions. The study also shows an increase in grain consumption and increasing dietary diversity through time, leading up to the well-known Mongolian Empire of the Khans.
Horses are still used by many for transport across Mongolia. Credits: Shevan Wilkin
The new discoveries presented in this paper show that the development of the earliest empires in Mongolia, like in other parts of the world, was tied to a diverse economy that included the local or regional production of grain. Dr. Bryan K. Miller, a co-author who studies the historical and archaeological records of Inner Asian empires, remarks that "these regimes were like most empires, in that they directed intricate political networks and sought to amass a stable surplus - in this case a primarily pastoral one that was augmented by other resources like millet."
"In this regard," Dr. Miller adds, "this study brings us one step closer to understanding the cultural processes that led humanity into the modern world."
The view that everyone in Mongolian history was a nomadic herder has skewed discussions concerning social development in this part of the world. Dr. Wilkin notes that "setting aside our preconceived ideas of what prehistory looked like and examining the archaeological record with modern scientific approaches is forcing us to rewrite entire sections of humanity's past." Dr. Spengler, the director of the archaeobotany labs at the MPI SHH, emphasizes the importance of this discovery, noting that "this study pulls the veil of myth and lore off of the real people who lived in Mongolia millennia ago and lets us peak into their lives."
Cultivated land in northern Mongolia. Credits: Alicia Ventresca Miller
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Publication information:
Title: Economic Diversification Supported the Growth of Mongolia's Nomadic Empires
Authors: Shevan Wilkin, Alicia Ventresca Miller, Bryan K. Miller, Robert N. Spengler, William T. T. Taylor, Ricardo Fernandes, Madeleine Bleasdale, Jana Zech, S. Ulziibayar, Erdene Myagmar, Nicole Boivin, Patrick Roberts
5,000-year-old milk proteins point to the importance of dairying in eastern Eurasia
Recent findings push back estimates of dairying in the eastern Steppe by more than 1,700 years, pointing to migration as a potential means of introduction
Today dairy foods sustain and support millions around the world, including in Mongolia, where dairy foods make up to 50% of calories consumed during the summer. Although dairy-based pastoralism has been an essential part of life and culture in the eastern Eurasian Steppe for millennia, the eastward spread of dairying from its origin in southwest Asia and the development of these practices is little understood. The current study, led by Shevan Wilkin and Jessica Hendy of the Max Planck Institute for the Science of Human History, presents the earliest evidence for dairy consumption in East Asia, circa 3000 BCE, and offers insights into the arrival and evolution of dairy pastoralism in prehistoric Mongolia.
These are horses on the steppe. Credits: Björn Reichhardt
Earliest dairy consumption & a possible path of entry
The highly mobile nature of pastoralist societies and the severe winds of the Eastern Steppe make detecting occupied sites with direct evidence into the lives and culture of ancient Mongolians exceedingly rare. Instead, the researchers looked for clues in ritual human burial mounds, often marked by stone monuments and occasionally featuring satellite animal graves.
In collaboration with the National University of Mongolia, researchers analyzed dental calculus from individuals ranging from the Early Bronze Age to the Mongol Period. Three-quarters of all individuals contained evidence that they had consumed dairy foods, which demonstrates the widespread importance of this food source in both prehistoric and historic Mongolia. The study's results include the earliest direct evidence for dairy consumption in East Asia, identified in an individual from the Afanasievo site of Shatar Chuluu, which dates to roughly 3000 BCE. Previous DNA analysis on this individual revealed non-local genetic markers consistent with Western Steppe Herder populations, presenting Early Bronze Age Afanasievo migrations westward via the Russian Altai as a viable candidate for the introduction of dairy and domestic livestock into eastern Eurasia.
Multiple different animal species were used for their milk
These are sheep and goat herds in Mongolia. Credits: Björn Reichhardt
By sequencing the milk proteins extracted from the dental calculus, the scientists were able to determine which animal species were being used for dairy production, and thereby help to trace the progression of domestication, dairying, and pastoralism in the region. "Modern Mongolians use cow, sheep, goat, yak, camel, horse and reindeer for milk today, yet when each of these species were first utilized for dairy in Mongolia remains unclear," says Shevan Wilkin, lead author of the study. "What is clear is that the crucial renewable calories and hydration made available through the incorporation of dairying would have become essential across the arid and agriculturally challenging ancient Eastern Steppe."
The earliest individuals to show evidence of dairy consumption lived around 5000 years ago and consumed milk from ruminant species, such as cattle, sheep, and goats. A few thousand years later, at Bronze Age sites dated to after 1200 BCE, the researchers find the first evidence of horse milk consumption, occurring at the same time as early evidence for horse bridling and riding, as well as the use of horses at ritual burial sites. In addition, the study shows that during the Mongol Empire circa 1200-1400 CE, people also consumed the milk of camels. "We are excited that through the analysis of proteins we are able to see the consumption of multiple different animal species, even sometimes in the same individual. This gives us a whole new insight into ancient dairying practices" says Jessica Hendy, senior author of the study.
Millenia after the first evidence of horse milk consumption, horses remain vital to the daily lives of many in modern Mongolia, where mounted pastoralists rely on them to manage large herds of livestock, transport people and supplies, and provide a primary source of meat and milk. "Our findings suggest that the incorporation of horses into dairy pastoralism in Eastern Eurasia was closely linked to a broader economic transformation in the use of horses for riding, movement, and diet," says William Taylor of the University of Colorado-Boulder, one of the study's coauthors.
Although the earliest individual sampled in this study showed evidence of dairy consumption, the researchers hope future studies will examine individuals from previous time periods. "In order to form a clearer picture of the origins of dairying in this region, we need to understand the impact of western steppe herder migrations and confirm whether dairying was occurring in Mongolia prior to their arrival," Shevan Wilkin concludes.
This is a horse burial at Morin Mort, Mongolia. Credits: William Taylor
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Publication information:
Title: Dairy pastoralism sustained Eastern Eurasian steppe populations for 5000 years
Authors: Shevan Wilkin, Alicia Ventresca Miller, William T.T. Taylor, Bryan K. Miller, Richard W. Hagan, Madeleine Bleasdale, Ashley Scott, Sumiya Gankhuyg, Abigail Ramsoe, S. Uliziibayar, Christian Trachsel, Paolo Nanni, Jonas Grossmann, Ludovic Orlando, Mark Horton, Philipp W. Stockhammer, Erdene Myagmar, Nicole Boivin, Christina Warinner, Jessica Hendy
Archaeologists find Bronze Age tombs lined with gold
The family tombs are near the 2015 site of the 'Griffin Warrior,' a military leader buried with armor, weapons and jewelry.
A gold ring depicts bulls and barley, the first known representation of domesticated animals and agriculture in ancient Greece. Credits: UC Classics
Archaeologists with the University of Cincinnati have discovered two Bronze Age tombs containing a trove of engraved jewelry and artifacts that promise to unlock secrets about life in ancient Greece.
The UC archaeologists announced the discovery Tuesday in Greece.
Jack Davis and Sharon Stocker, archaeologists in UC's classics department, found the two beehive-shaped tombs in Pylos, Greece, last year while investigating the area around the grave of an individual they have called the "Griffin Warrior," a Greek man whose final resting place they discovered nearby in 2015.
Like the Griffin Warrior's tomb, the princely tombs overlooking the Mediterranean Sea also contained a wealth of cultural artifacts and delicate jewelry that could help historians fill in gaps in our knowledge of early Greek civilization.
UC's team spent more than 18 months excavating and documenting the find. The tombs were littered with flakes of gold leaf that once papered the walls.
"Like with the Griffin Warrior grave, by the end of the first week we knew we had something that was really important," said Stocker, who supervised the excavation.
"It soon became clear to us that lightning had struck again," said Davis, head of UC's classics department.
UC archaeologists discovered two large family tombs at Pylos, Greece, strewn with flakes of gold that once lined their walls. The excavation took more than 18 months. Credits: UC Classics
The Griffin Warrior is named for the mythological creature -- part eagle, part lion -- engraved on an ivory plaque in his tomb, which also contained armor, weaponry and gold jewelry. Among the priceless objects of art was an agate sealstone depicting mortal combat with such fine detail that Archaeology magazine hailed it as a "Bronze Age masterpiece."
Artifacts found in the princely tombs tell similar stories about life along the Mediterranean 3,500 years ago, Davis said. A gold ring depicted two bulls flanked by sheaves of grain, identified as barley by a paleobotanist who consulted on the project.
"It's an interesting scene of animal husbandry -- cattle mixed with grain production. It's the foundation of agriculture," Davis said. "As far as we know, it's the only representation of grain in the art of Crete or Minoan civilization."
UC archaeologists found a sealstone made from semiprecious carnelian in the family tombs at Pylos, Greece. The sealstone was engraved with two lionlike mythological figures called genii carrying serving vessels and incense burners facing each other over an altar and below a 16-pointed star. The other image is a putty cast of the sealstone. Credits: UC Classics
Like the grave of the Griffin Warrior, the two family tombs contained artwork emblazoned with mythological creatures. An agate sealstone featured two lion-like creatures called genii standing upright on clawed feet. They carry a serving vase and an incense burner, a tribute for the altar before them featuring a sprouting sapling between horns of consecration, Stocker said.
Above the genii is a 16-pointed star. The same 16-pointed star also appears on a bronze and gold artifact in the grave, she said.
"It's rare. There aren't many 16-pointed stars in Mycenaean iconography. The fact that we have two objects with 16 points in two different media (agate and gold) is noteworthy," Stocker said.
The genius motif appears elsewhere in the East during this period, she said.
"One problem is we don't have any writing from the Minoan or Mycenaean time that talks of their religion or explains the importance of their symbols," Stocker said.
UC's team also found a gold pendant featuring the likeness of the Egyptian goddess Hathor.
"Its discovery is particularly interesting in light of the role she played in Egypt as protectress of the dead," Davis said.
The identity of the Griffin Warrior is a matter for speculation. Stocker said the combination of armor, weapons and jewelry found in his tomb strongly indicate he had military and religious authority, likely as the king known in later Mycenaean times as a wanax.
Likewise, the princely tombs paint a picture of accumulated wealth and status, she said. They contained amber from the Baltic, amethyst from Egypt, imported carnelian and lots of gold. The tombs sit on a scenic vista overlooking the Mediterranean Sea on the spot where the Palace of Nestor would later rise and fall to ruins.
"I think these are probably people who were very sophisticated for their time," she said. "They have come out of a place in history where there were few luxury items and imported goods. And all of a sudden at the time of the first tholos tombs, luxury items appear in Greece.
"You have this explosion of wealth. People are vying for power," she said. "It's the formative years that will give rise to the Classic Age of Greece."
The antiquities provide evidence that coastal Pylos was once an important destination for commerce and trade.
"If you look at a map, Pylos is a remote area now. You have to cross mountains to get here. Until recently, it hasn't even been on the tourist path," Stocker said. "But if you're coming by sea, the location makes more sense. It's on the way to Italy. What we're learning is that it's a much more central and important place on the Bronze Age trade route."
The princely tombs sit close to the palace of Nestor, a ruler mentioned in Homer's famous works "The Iliad" and "The Odyssey." The palace was discovered in 1939 by the late UC Classics professor Carl Blegen. Blegen had wanted to excavate in the 1950s in the field where Davis and Stocker found the new tombs but could not get permission from the property owner to expand his investigation. The tombs would have to wait years for another UC team to make the startling discovery hidden beneath its grape vines.
Excavating the site was particularly arduous. With the excavation season looming, delays in procuring the site forced researchers to postpone plans to study the site first with ground-penetrating radar. Instead, Stocker and Davis relied on their experience and intuition to focus on one disturbed area.
"There were noticeable concentrations of rocks on the surface once we got rid of the vegetation," she said.
Those turned out to be the exposed covers of deep tombs, one plunging nearly 15 feet. The tombs were protected from the elements and potential thieves by an estimated 40,000 stones the size of watermelons.
The boulders had sat undisturbed for millennia where they had fallen when the domes of the tombs collapsed. And now 3,500 years later, UC's team had to remove each stone individually.
"It was like going back to the Mycenaean Period. They had placed them by hand in the walls of the tombs and we were taking them out by hand," Stocker said. "It was a lot of work."
At every step of the excavation, the researchers used photogrammetry and digital mapping to document the location and orientation of objects in the tomb. This is especially valuable because of the great number of artifacts that were recovered, Davis said.
"We can see all levels as we excavated them and relate them one to the other in three dimensions," he said. UC's team will continue working at Pylos for at least the next two years while they and other researchers around the world unravel mysteries contained in the artifacts.
"It has been 50 years since any substantial tombs of this sort have been found at any Bronze Age palatial site. That makes this extraordinary," Davis said.
Press release from the University of Cincinnati, by Michael Miller.
Lost in Combat? Researchers discover belongings of a warrior on unique Bronze Age battlefield site
This collection of objects was found by divers in the Tollense river and is probably the contents of a personal pouch of a warrior who died 3,300 years ago on the battlefield. Credit: Volker Minkus
Recent archaeological investigations in the Tollense Valley led by the University of Göttingen, the State Agency for Cultural Heritage in Mecklenburg-Vorpommern and the University of Greifswald have unearthed a collection of 31 unusual objects. Researchers believe this is the personal equipment of a Bronze Age warrior who died on the battlefield 3,300 years ago. This unique find was discovered by a diving team headed by Dr Joachim Krüger, from the University of Greifswald, and seems to have been protected in the river from the looting, which inevitably followed fighting. The study was published in Antiquity.
These are the battlefield remains from the layer where objects were found at the site near the Tollense river in Weltzin. Credit: Stefan Sauer
The archaeological records of the European Bronze Age are dominated by settlement finds, hoards and evidence of funeral sites. However, the site at the river Tollense in Northern Germany is very different and provides for the first time in Europe the evidence of a prehistoric battlefield. Over 12,000 pieces of human bone have already been recovered from the valley and osteoanthropologist Ute Brinker, from the State Agency has identified more than 140 individuals – young adult males in good physical condition. Their bones showed signs of recent trauma – the result of close and long-range weapons – and healed lesions, which probably indicate they were accustomed to combat. Isotopic results suggested that at least some of the group were not from the local area, but until now, it was not clear how far they travelled.
The discovery of a new set of artefacts from the remains of battle provides important new clues. The divers could document a number of Bronze finds in their original position on the river ground, among them a decorated belt box, three dress pins and also arrow heads. Surprisingly they also found 31 objects (250g) tightly packed together, suggesting they were in a container made of wood or cloth that has since rotted away. The items include a bronze tool with a birch handle, a knife, a chisel and fragments of bronze. Radiocarbon dating of the collection of objects demonstrates that the finds belong to the battlefield layer and they were probably the personal equipment of one of the victims. The finds were studied in a Master’s thesis by Tobias Uhlig and the new results make it increasingly clear that there was a massive violent conflict in the older Nordic Bronze Age (2000–1200 BC). In fact, recent evidence suggests that it is likely to have been on a large scale, clearly stretching beyond regional borders.
This is a human skull found in the Tollense valley with fatal trauma caused by a Bronze arrowhead. Credit: Volker Minkus
Professor Thomas Terberger, from the Department of Pre- and Early History at the University of Göttingen, says, “This is the first discovery of personal belongings on a battlefield and it provides insights into the equipment of a warrior. The fragmented bronze was probably used as a form of early currency. The discovery of a new set of artefacts also provides us with clues about the origins of the men who fought in this battle and there is increasing evidence that at least some of the warriors originated in southern Central Europe.”
Original publication: Tobias Uhlig et al. Lost in combat? A scrap metal find from the Bronze Age battlefield site at Tollense(2019), Antiquity. DOI: 10.15184/aqy.2019.137
